other:
Chromatin Immunoprecipitation:Article Title: ZMYND8 expression in breast cancer cells blocks T-lymphocyte surveillance to promote tumor growth
Article Snippet: .. The supernatant was diluted in ChIP immunoprecipitation buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail) and then subjected to immunoprecipitation overnight in the presence of Dynabeads (ThermoFisher) with anti-phospho-p65 (Ser536) antibody (3033S, Cell Signaling Technology) or normal rabbit IgG (2729S, Cell Signaling Technology) at 4 °C. ..
Article Title: ZMYND8 expression in breast cancer cells blocks T-lymphocyte surveillance to promote tumor growth
Article Snippet: .. The supernatant was diluted in ChIP immunoprecipitation buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail) and then subjected to immunoprecipitation overnight in the presence of Dynabeads (ThermoFisher) with anti-phospho-p65 (Ser536) antibody (3033S, Cell Signaling Technology) or normal rabbit IgG (2729S, Cell Signaling Technology) at 4 °C. ..
Article Title: HIF-activated priming of TRAIL-induced cell death determines epigenetic vulnerability in kidney cancer
Article Snippet: Cells were stained with PI and Hoechst for 10 min at room temperature and imaged with Zeiss Axio Observer Z1 fluorescence microscope. .. The supernatant was diluted in ChIP immunoprecipitation buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail) and then subjected to immunoprecipitation overnight in the presence of Dynabeads (ThermoFisher) with anti-HIF-1α antibody (3 μg, home-made), anti-HIF-2α antibody (3 μg, home-made), anti-DNMT1 antibody (3 μg, 24206-1-AP, Proteintech), or anti-DNMT3A antibody (3 μg, 20954-1-AP, Proteintech), anti-DNMT3B antibody (3 μg, 26971-1-AP, Proteintech), or normal rabbit IgG (3 μg, 2729S, Cell Signaling Technology) at 4°C. .. To validate cell death inhibitors, RCC10 (1 × 10 5 cells/well) cells were seeded onto the 12-well plate for 12 h and pre-treated for 30 min with vehicle, Necrostatin-1 (10 μM), Ferrostatin-1 (2 μM), 3-MA (1.25 mM), or AC-FLTD-CMK (10 μM), followed by 3 days of co-treatment with vehicle or respective cell death activator, including Compound6i (0.5 μM, necroptosis activator, HY-144828, MCE), RSL3 (50 nM, ferroptosis activator, HY-100218A, MCE), STF-62247 (5 μM, autophagy inducer, HY-100746, MCE), or Nigericin (1 μM, pyroptosis inducer, HY-127019, MCE).
Article Title: ZMYND8 drives HER2 antibody resistance in breast cancer via lipid control of IL-27.
Article Snippet: .. The supernatant was diluted in ChIP immunoprecipitation buffer (50mM HEPES-KOH, pH 7.5, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail) and then subjected to immunoprecipitation overnight in the presence of Dynabeads (ThermoFisher) with antic-Myc antibody (sc-40, Santa Cruz Biotechnology) or normal mouse IgG (sc-2025, Santa Cruz Biotechnology) at 4 °C. .. Beads were washed, eluted, and reverse-crosslinked at 65 °C for 4 h, followed by treatment with proteinase K at 45 °C for 1 h. The precipitated DNA was purified withMinElute PCR purification kit (Qiagen) and quantified by real-time qPCR assay.
Article Title: ZMYND8 drives HER2 antibody resistance in breast cancer via lipid control of IL-27
Article Snippet: .. The supernatant was diluted in ChIP immunoprecipitation buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail) and then subjected to immunoprecipitation overnight in the presence of Dynabeads (ThermoFisher) with anti-c-Myc antibody (sc-40, Santa Cruz Biotechnology) or normal mouse IgG (sc-2025, Santa Cruz Biotechnology) at 4 °C. .. Beads were washed, eluted, and reverse-crosslinked at 65 °C for 4 h, followed by treatment with proteinase K at 45 °C for 1 h. The precipitated DNA was purified with MinElute PCR purification kit (Qiagen) and quantified by real-time qPCR assay.
Immunoprecipitation:Article Title: ZMYND8 expression in breast cancer cells blocks T-lymphocyte surveillance to promote tumor growth
Article Snippet: .. The supernatant was diluted in ChIP immunoprecipitation buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail) and then subjected to immunoprecipitation overnight in the presence of Dynabeads (ThermoFisher) with anti-phospho-p65 (Ser536) antibody (3033S, Cell Signaling Technology) or normal rabbit IgG (2729S, Cell Signaling Technology) at 4 °C. ..
Article Title: ZMYND8 expression in breast cancer cells blocks T-lymphocyte surveillance to promote tumor growth
Article Snippet: .. The supernatant was diluted in ChIP immunoprecipitation buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail) and then subjected to immunoprecipitation overnight in the presence of Dynabeads (ThermoFisher) with anti-phospho-p65 (Ser536) antibody (3033S, Cell Signaling Technology) or normal rabbit IgG (2729S, Cell Signaling Technology) at 4 °C. ..
Article Title: HIF-activated priming of TRAIL-induced cell death determines epigenetic vulnerability in kidney cancer
Article Snippet: Cells were stained with PI and Hoechst for 10 min at room temperature and imaged with Zeiss Axio Observer Z1 fluorescence microscope. .. The supernatant was diluted in ChIP immunoprecipitation buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail) and then subjected to immunoprecipitation overnight in the presence of Dynabeads (ThermoFisher) with anti-HIF-1α antibody (3 μg, home-made), anti-HIF-2α antibody (3 μg, home-made), anti-DNMT1 antibody (3 μg, 24206-1-AP, Proteintech), or anti-DNMT3A antibody (3 μg, 20954-1-AP, Proteintech), anti-DNMT3B antibody (3 μg, 26971-1-AP, Proteintech), or normal rabbit IgG (3 μg, 2729S, Cell Signaling Technology) at 4°C. .. To validate cell death inhibitors, RCC10 (1 × 10 5 cells/well) cells were seeded onto the 12-well plate for 12 h and pre-treated for 30 min with vehicle, Necrostatin-1 (10 μM), Ferrostatin-1 (2 μM), 3-MA (1.25 mM), or AC-FLTD-CMK (10 μM), followed by 3 days of co-treatment with vehicle or respective cell death activator, including Compound6i (0.5 μM, necroptosis activator, HY-144828, MCE), RSL3 (50 nM, ferroptosis activator, HY-100218A, MCE), STF-62247 (5 μM, autophagy inducer, HY-100746, MCE), or Nigericin (1 μM, pyroptosis inducer, HY-127019, MCE).
Article Title: ZMYND8 drives HER2 antibody resistance in breast cancer via lipid control of IL-27.
Article Snippet: .. The supernatant was diluted in ChIP immunoprecipitation buffer (50mM HEPES-KOH, pH 7.5, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail) and then subjected to immunoprecipitation overnight in the presence of Dynabeads (ThermoFisher) with antic-Myc antibody (sc-40, Santa Cruz Biotechnology) or normal mouse IgG (sc-2025, Santa Cruz Biotechnology) at 4 °C. .. Beads were washed, eluted, and reverse-crosslinked at 65 °C for 4 h, followed by treatment with proteinase K at 45 °C for 1 h. The precipitated DNA was purified withMinElute PCR purification kit (Qiagen) and quantified by real-time qPCR assay.
Article Title: ZMYND8 drives HER2 antibody resistance in breast cancer via lipid control of IL-27
Article Snippet: .. The supernatant was diluted in ChIP immunoprecipitation buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail) and then subjected to immunoprecipitation overnight in the presence of Dynabeads (ThermoFisher) with anti-c-Myc antibody (sc-40, Santa Cruz Biotechnology) or normal mouse IgG (sc-2025, Santa Cruz Biotechnology) at 4 °C. .. Beads were washed, eluted, and reverse-crosslinked at 65 °C for 4 h, followed by treatment with proteinase K at 45 °C for 1 h. The precipitated DNA was purified with MinElute PCR purification kit (Qiagen) and quantified by real-time qPCR assay.
Protease Inhibitor:Article Title: ZMYND8 expression in breast cancer cells blocks T-lymphocyte surveillance to promote tumor growth
Article Snippet: .. The supernatant was diluted in ChIP immunoprecipitation buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail) and then subjected to immunoprecipitation overnight in the presence of Dynabeads (ThermoFisher) with anti-phospho-p65 (Ser536) antibody (3033S, Cell Signaling Technology) or normal rabbit IgG (2729S, Cell Signaling Technology) at 4 °C. ..
Article Title: ZMYND8 expression in breast cancer cells blocks T-lymphocyte surveillance to promote tumor growth
Article Snippet: .. The supernatant was diluted in ChIP immunoprecipitation buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail) and then subjected to immunoprecipitation overnight in the presence of Dynabeads (ThermoFisher) with anti-phospho-p65 (Ser536) antibody (3033S, Cell Signaling Technology) or normal rabbit IgG (2729S, Cell Signaling Technology) at 4 °C. ..
Article Title: HIF-activated priming of TRAIL-induced cell death determines epigenetic vulnerability in kidney cancer
Article Snippet: Cells were stained with PI and Hoechst for 10 min at room temperature and imaged with Zeiss Axio Observer Z1 fluorescence microscope. .. The supernatant was diluted in ChIP immunoprecipitation buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail) and then subjected to immunoprecipitation overnight in the presence of Dynabeads (ThermoFisher) with anti-HIF-1α antibody (3 μg, home-made), anti-HIF-2α antibody (3 μg, home-made), anti-DNMT1 antibody (3 μg, 24206-1-AP, Proteintech), or anti-DNMT3A antibody (3 μg, 20954-1-AP, Proteintech), anti-DNMT3B antibody (3 μg, 26971-1-AP, Proteintech), or normal rabbit IgG (3 μg, 2729S, Cell Signaling Technology) at 4°C. .. To validate cell death inhibitors, RCC10 (1 × 10 5 cells/well) cells were seeded onto the 12-well plate for 12 h and pre-treated for 30 min with vehicle, Necrostatin-1 (10 μM), Ferrostatin-1 (2 μM), 3-MA (1.25 mM), or AC-FLTD-CMK (10 μM), followed by 3 days of co-treatment with vehicle or respective cell death activator, including Compound6i (0.5 μM, necroptosis activator, HY-144828, MCE), RSL3 (50 nM, ferroptosis activator, HY-100218A, MCE), STF-62247 (5 μM, autophagy inducer, HY-100746, MCE), or Nigericin (1 μM, pyroptosis inducer, HY-127019, MCE).
Article Title: ZMYND8 drives HER2 antibody resistance in breast cancer via lipid control of IL-27.
Article Snippet: .. The supernatant was diluted in ChIP immunoprecipitation buffer (50mM HEPES-KOH, pH 7.5, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail) and then subjected to immunoprecipitation overnight in the presence of Dynabeads (ThermoFisher) with antic-Myc antibody (sc-40, Santa Cruz Biotechnology) or normal mouse IgG (sc-2025, Santa Cruz Biotechnology) at 4 °C. .. Beads were washed, eluted, and reverse-crosslinked at 65 °C for 4 h, followed by treatment with proteinase K at 45 °C for 1 h. The precipitated DNA was purified withMinElute PCR purification kit (Qiagen) and quantified by real-time qPCR assay.
Article Title: ZMYND8 drives HER2 antibody resistance in breast cancer via lipid control of IL-27
Article Snippet: .. The supernatant was diluted in ChIP immunoprecipitation buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail) and then subjected to immunoprecipitation overnight in the presence of Dynabeads (ThermoFisher) with anti-c-Myc antibody (sc-40, Santa Cruz Biotechnology) or normal mouse IgG (sc-2025, Santa Cruz Biotechnology) at 4 °C. .. Beads were washed, eluted, and reverse-crosslinked at 65 °C for 4 h, followed by treatment with proteinase K at 45 °C for 1 h. The precipitated DNA was purified with MinElute PCR purification kit (Qiagen) and quantified by real-time qPCR assay.
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